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Nitrate Nitrogen Quantification via Ultraviolet Absorbance: A Case Study in Agricultural and Horticultural Regions in Central China
Yiheng Zang 
,
Jing Chen,
Muhammad Awais,
Mukhtar Iderawumi Abdulraheem ,
Moshood Abiodun Yusuff ,
Kuan Geng,
Yongqi Chen,
Yani Xiong,
Linze Li,
Yanyan Zhang,
Vijaya Raghavan ,
Jiandong Hu ,
Junfeng Wu,
Guoqing Zhao
,
Jing Chen,
Muhammad Awais,
Mukhtar Iderawumi Abdulraheem ,
Moshood Abiodun Yusuff ,
Kuan Geng,
Yongqi Chen,
Yani Xiong,
Linze Li,
Yanyan Zhang,
Vijaya Raghavan ,
Jiandong Hu ,
Junfeng Wu,
Guoqing Zhao
Agriculture, Volume: 15, Issue: 11, Start page: 1131
Swansea University Author:
Guoqing Zhao
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.3390/agriculture15111131
Abstract
Soil nitrate nitrogen (NO3−-N) is a key indicator of agricultural non-point source pollution. The ultraviolet (UV) dual-wavelength method is widely used for NO3−-N detection, but interference from complex soil organic matter affects its accuracy. This study investigated how organic matter influences...
| Published in: | Agriculture |
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| ISSN: | 2077-0472 |
| Published: |
MDPI AG
2025
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa69670 |
| Abstract: |
Soil nitrate nitrogen (NO3−-N) is a key indicator of agricultural non-point source pollution. The ultraviolet (UV) dual-wavelength method is widely used for NO3−-N detection, but interference from complex soil organic matter affects its accuracy. This study investigated how organic matter influences NO3−-N detection by optimizing UV dual-wavelength combinations. Density functional theory (DFT) calculations showed slight spectral broadening of fulvic and humic acids in the presence of NO3−-N under UV spectrum. Standard solutions and soil samples were used to compare the detection performance of different wavelength pairs. The findings indicated that the dual-wavelength combination of 235 nm/275 nm is optimal rather than 220 nm/275 nm for measuring soil samples at NO3−-N concentrations exceeding 5 mg·L−1. The 235/275 nm method gave an average calibration coefficient of 1.57. Compared to the national standard and flow analysis methods, the average relative errors were 19.7% and 22.3% (p < 0.001), respectively, indicating its suitability for practical soil applications. These results demonstrate the method’s potential for rapid and accurate NO3−-N detection in real soil samples, supporting its application in environmental monitoring and agricultural management. |
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| Keywords: |
nitrate nitrogen in soil; ultraviolet dual-wavelength; agricultural non-point source pollution (ANPS); soil organic matter; calibration coefficient |
| College: |
Faculty of Humanities and Social Sciences |
| Funders: |
This work was supported by the 14th Five-Year National Key Research and Development Program (2024YFD17000802, 2021YFD1700904), the Major Science and Technology Projects of Henan Province (221111320700), and Henan Center for Outstanding Overseas Scientists (GZS2021007). |
| Issue: |
11 |
| Start Page: |
1131 |